Small Molecule Profiling and Assay Development
Our custom assay services can help to accelerate your small molecule drug discovery and development workflow. These comprehensive services are built around award-winning bioluminescent technology for sensitive, high-throughput and biologically relevant results.
Targeted Protein Degradation/CRISPR Knock-in
The combination of CRISPR/Cas9 knock-in and live-cell bioluminescent detection methods offers a novel approach to study endogenous biology. Changes in protein abundance can be monitored in real time to better understand cellular protein dynamics.
SERVICES INCLUDE
- Custom knock-in cell line development; learn more about available cell lines
- Protein degradation profiling with optional cell viability monitoring
NanoBRET™ Target Engagement Assays
The NanoBRET™ Target Engagement Assays are live-cell binding assays that use an energy transfer technique to measure molecular proximity. These assays also provide information about compound-target residence time and can be used to screen a panel of inhibitors to compare IC50 values.
SERVICES INCLUDE
- Tracer synthesis (from total synthesis to dye conjugation of target molecules)
- Assay development for novel protein targets
- Compound profiling of existing targets (e.g., CRBN, VHL, RAS, PARP)
- Cellular permeability
- Thaw-and-Use (assay-ready) cells
Compound Profiling Services
Get more biologically relevant results from your early-stage drug discovery screening. Rely on proven, highly sensitive luminescent assay readouts for target identification, validation and lead optimization. Test your compounds against any Promega assay!
SERVICES INCLUDE
- Selectivity profiling in live cells, using NanoBRET™ Target Engagement (TE) against 192 or 234 kinase proteins with dose-response follow-ups
- Custom profiling for kinases not in the standard panels
- Protein degradation profiling with optional cell viability monitoring
- NanoBRET™ TE Assays–RAS, NLRP3, PARPs
- Protein-protein interaction assays
Protein:Protein Interaction (PPI) Assays
The bright luminescence and small size of NanoLuc® Luciferase allows sensitive detection of protein interactions in live cells using BRET (NanoBRET™) or complementation reporter-based (NanoBiT®) methods. These assays enable detection of protein interactions at endogenous expression levels in high-throughput, multiwell plate formats.
SERVICES INCLUDE
- NanoBRET™ PPI Assays: Vectors, assay optimization, stable cell line development, compound testing
- NanoBiT® PPI Assays: Vectors, assay optimization, stable cell line development, compound testing
Lumit™ Technology for Immunoassays
Lumit™ Immunoassays provide a simple and fast alternative to conventional immunoassay methods, including sandwich ELISAs and Western blots. The bioluminescence-based method can be completed in as little as 30 minutes.
SERVICES INCLUDE
- SmBiT® and LgBiT® antibody labeling for X-Y antibodies that can be used to determine the optimal Ab pair for an immunoassay
- Bulk labeling of selected antibodies from 1mg to 10mg
- Testing primary Ab pairs with our pre-labeled secondary Ab detection system
- Custom immunoassay development
Reporter Gene Assays
Reporter gene assays can be used to investigate a variety of cellular processes using bioluminescent light output as a sensitive measure of biological activity. We offer a full range of assay products and tools for your research.
SERVICES INCLUDE
- Reporter vectors
- Assay optimization
- Stable cell line development
- Compound testing